The HAEC is separated from the placenta at the Cesarean section on the basis of the informed consent through the known procudures and cultured in the RPMI medium including 10% FCS until the HAEC is sufficiently proliferated. インフォームドコンセントに基づいて帝王切開時に得られた胎盤からHAECを公知の方法により分離し、10%FCS含有RPMI培地で培養し、HAECを十分に増殖させた。 - 特許庁
Subsequently, the proliferated HAEC is washed with PBS, and cultured in DMEM including 0.5% FCS for 48 hours and the supernatant is collected therefrom. 続いて、PBSで洗浄後、0.5%FCS含有DMEMで48時間培養し、上清を回収した。 - 特許庁