An unknown human Nek kinase family member is isolated by cloning a gene expressed in an extremely small amount by using a method comprising the combination of RCE method and Nested-PCR method and its base sequence and the amino acid sequence are determined. 極微量にしか発現していない遺伝子をRACE法とNested-PCR法を組み合わせた方法により、ヒトの未知Nekキナーゼファミリーメンバーを単離し、その塩基配列とアミノ酸配列を決定した。 - 特許庁