The synthesis of the complementalstrand on the untargeted base sequence is prevented by using an oligonucleotide capable of specifically annealing to a mutation site on the untargeted base sequence. 非標的塩基配列上の変異部位に特異的にアニールしうるオリゴヌクレオチドを用いて、該非標的塩基配列上からの相補鎖合成を防止する。 - 特許庁
To provide a method for detecting mutation by which the mutation is more universally and accurately detected in good sensitivity while precisely preventing the synthesis of a complementalstrand on an untargeted base sequence even in any nucleic acid sequence in the method for detecting the mutation of a gene by using a LAMP method. LAMP法を用いた遺伝子の変異検出方法において、非標的塩基配列上からの相補鎖合成をどのような核酸配列においても確実に防止して、より普遍的に、正確かつ感度の良い変異検出方法を提供すること。 - 特許庁
This molecular beacon by utilizing the switching of the monomeric light emission and excimer light emission is provided by bonding a fluorescent organic group capable of forming an excimer with both 3' and 5' terminals of a single strand oligonucleotide to be hybridized with an oligonucleotide to be tested, and the method for detecting a complemental chain DNA sequence and the method for detecting an SNP by using the molecular beacon are also provided. 被検オリゴヌクレオチドとハイブリダイズさせる一本鎖オリゴヌクレオチドの3'及び5'両末端にエキシマー形成可能な蛍光性有機基を結合させ、モノマー発光とエキシマー発光のスイッチングを利用した分子ビーコン、及び該分子ビーコンを用いる相補鎖DNA配列検出方法ならびにSNP検出方法。 - 特許庁