Wiktionary英語版

出典:Wiktionary

ponceaus

出典:『Wiktionary』 (2010/09/06 21:32 UTC 版)

名詞

ponceaus

  1. Plural form of ponceau.
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ウィキペディア英語版

出典:Wikipedia

Ponceau S

出典:『Wikipedia』 (2011/05/06 12:24 UTC 版)

英語による解説

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Ponceau S, Acid Red 112, or C.I. 27195 is a sodium salt of a diazo dye that may be used to prepare a stain for rapid reversible detection of protein bands on nitrocellulose or PVDF membranes (Western blotting), as well as on cellulose acetate membranes. A Ponceau S stain is is useful because it does not appear to have a deleterious effect on the sequencing of blotted polypeptides and is therefore one method of choice for locating polypeptides on Western blots for blot-sequencing. It is also easily reversed with water washes, facilitating subsequent immunological detection. The stain can be completely removed from the protein bands by continued washing. Common stain formulations include 0.1% (w/v) Ponceau S in 5% acetic acid or 2% (w/v) Ponceau S in 30% TCA and 30% sulfosalicylic acid.

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ponceaus
dedeaux field
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