MTSLとは 意味・読み方・使い方

MTSL (S-(2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl methanesulfonothioate) is a chemical compound which can be used as a nitroxide (amine oxide) paramagnetic spin label in protein Electron paramagnetic resonance spectroscopy experiments. MTSL is attached via a disulfide bond to a cysteine residue, enabling site-directed spin labelling. Following attachment, which involves a Sulfinic acid (CH₃SO₂) leaving group, the MTSL moiety will add 186.3 daltons to the mass of the protein or peptide to which it is attached. The cysteine can be introduced using site-directed mutagenesis, and hence most positions in a protein can be labelled.