Wiktionary英語版

出典：Wiktionary

blue-white screen

出典:『Wiktionary』 (2015/08/29 08:27 UTC 版)

名詞

blue-white screen ‎(複数形 blue-white screens)

  1. (biology) A screening technique for the rapid detection of recombinant bacteria in vector-based molecular cloning experiments. DNA of interest is ligated into a vector, which is then inserted into a host cell; these are grown in the presence of X-gal. Cells transformed with vectors containing recombinant DNA will produce white colonies; cells transformed with non-recombinant plasmids (i.e. only the vector) grow into blue colonies.
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ウィキペディア英語版

出典：Wikipedia

Blue white screen

出典:『Wikipedia』 (2011/06/23 17:47 UTC 版)

英語による解説

ウィキペディア英語版からの引用
引用

The blue-white screen is a molecular technique that allows for the detection of successful ligations in vector-based gene cloning. DNA of interest is ligated into a vector. The vector is then transformed into competent cell (bacteria). The competent cells are grown in the presence of X-gal. If the ligation was successful, the bacterial colony will be white; if not, the colony will be blue. This technique allows for the quick and easy detection of successful ligation, without the need to individually test each colony. An example of such a vector is the artificially reconstructed plasmid pUC19.

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