出典:Wiktionary
出典:Wikipedia
出典:『Wikipedia』 (2011/07/03 15:27 UTC 版)
Pyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by synthesis" principle. It differs from Sanger sequencing, in that it relies on the detection of pyrophosphate release on nucleotide incorporation, rather than chain termination with dideoxynucleotides. The technique was developed by Pål Nyrén and Mostafa Ronaghi at the Royal Institute of Technology in Stockholm in 1996.