英和和英

出典:『Wikipedia』 (2011/03/30 23:18 UTC 版)

英語による解説

引用

TA cloning is a subcloning technique that doesn't use restriction enzymes and is easier and quicker than traditional subcloning. The technique relies on the ability of adenine (A) and thymine (T) (complementary basepairs) on different DNA fragments to hybridize and, in the presence of ligase, become ligated together. PCR products are usually amplified using Taq DNA polymerase which preferentially adds an adenine to the 3' end of the product. Such PCR amplified inserts are cloned into linearized vectors that have complementary 3' thymine overhangs. Commercialized kits with pre-prepared vectors and PCR reagents are currently sold, greatly speeding up the process.