出典:Wiktionary
出典:『Wiktionary』 (2015/12/29 01:14 UTC 版)
From recombinogenic + engineering.
recombineering (uncountable)
出典:Wikipedia
出典:『Wikipedia』 (2011/08/01 18:15 UTC 版)
Recombineering (recombination-mediated genetic engineering) is a genetic and molecular biology technique based on homologous recombination systems, as opposed to the older/more common method of using restriction enzymes and ligases to cut and glue DNA sequences. It has been developed in E. coli and now is expanding to other bacteria species and is used to modify DNA in a precise and simple manner. The procedure is widely used for bacterial genetics, in the generation of target vectors for making a conditional mouse knockout, and for modifying DNA of any source often contained on a bacterial artificial chromosome (BAC).