出典:Wikipedia
出典:『Wikipedia』 (2011/04/29 14:58 UTC 版)
A plot of B/F (bound ligand/free ligand) versus B is known as a Scatchard plot. It is a method for analyzing data for freely reversible ligand/receptor binding interactions. The plot yields a straight line of slope -1/K, where K is the ligand concentration at which the receptor is half-maximally occupied by ligand. The intercept on the B axis is Bmax. It is sometimes the case that binding data does not form a straight line when plotted in a Scatchard plot. Such is the case when ligand bound to substrate is not allowed to achieve equilibrium before the binding is measured.