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出典:Wikipedia
出典:『Wikipedia』 (2011/07/01 12:45 UTC 版)
Reversed-phase chromatography (RPC) includes any chromatographic method that uses a non-polar stationary phase. The name "reversed phase" has a historical background. In the 1970s most liquid chromatography was done on non-modified silica or alumina with a hydrophilic surface chemistry and a stronger affinity for polar compounds - hence it was considered "normal". The introduction of alkyl chains bonded covalently to the support surface reversed the elution order . Now in RPC, polar compounds are eluted first while non-polar compounds are retained - hence "reversed phase". All of the mathematical and experimental considerations used in other chromatographic methods apply (i.e. separation resolution proportional to the column length). Today, reversed-phase column chromatography accounts for the vast majority of analysis performed in liquid chromatography.