insertion deletion mutationsとは 意味・読み方・使い方

To provide a technique for the Gateway cloning of a large-size gene for which a PCR amplification becomes difficult and an error probability in the amplification becomes higher and for a cloning by introducing mutations of point mutation, deletion, insertion and substitution into a gene in high accuracy and efficiency.例文帳に追加

ＰＣＲ増幅が困難となり増幅の際のエラー率が高くなるサイズの大きな遺伝子のＧａｔｅｗａｙクローニングと遺伝子内に高精度高効率で点変異、欠失、挿入、置換という変異を導入しクローニングを行う技術を提供する。 - 特許庁

(A) A protein having a specific amino acid sequence and (B) a protein having the 20th amino acid residue that is alanine in the specific amino acid sequence and an amino acid sequence containing one or several mutations of amino acids selected from the group consisting of substitution, deletion, insertion, addition and inversion in a region other than the alanine and the peptide production activity.例文帳に追加

（Ａ）特定のアミノ酸配列を有するタンパク質（Ｂ）特定のアミノ酸配列における第２０番目のアミノ酸残基がアラニンであり、当該アラニン以外の領域に、置換、欠失、挿入、付加および逆位からなる群より選ばれる１または数個のアミノ酸の変異を含むアミノ酸配列を有し、かつ、ペプチド生成活性を有するタンパク質。 - 特許庁

Disclosed are a protein having a specific amino acid sequence and DNA replication activity, a protein having an amino acid sequence including one or several amino acid mutations selected from the group consisting of substitution, deletion, insertion and addition in a region except a substitution site in the protein as well as having DNA replication activity, a polynucleotide encoding the protein, a transformant introduced with the polynucleotide and uses thereof.例文帳に追加

特定のアミノ酸配列を有し、ＤＮＡ複製活性を有するタンパク質、または前記タンパク質における置換部位以外の領域に置換、欠失、挿入および付加からなる群より選ばれる１または数個のアミノ酸の変異を含むアミノ酸配列を有し、かつ、ＤＮＡ複製活性を有するタンパク質、該タンパク質をコードするポリヌクレオチド、該ポリヌクレオチドが導入された形質転換体及びこれらの用途。 - 特許庁