出典:Wikipedia
出典:『Wikipedia』 (2011/01/21 13:19 UTC 版)
Differential display, also referred to as DDRT-PCR or DD-PCR in PCR nomenclature, is the technique where a researcher can identify and analyze altered gene expression at the mRNA level in any eukaryotic cell. A researcher will study at least two samples, but many more can be studied if the experiment suggests so. These samples will have morphological, genetic or other experimental differences for which the researcher wishes to study the gene expression patterns, hoping to elucidate the root cause of the particular difference or specific genes that are affected by the experiment. Samples can be from any eukaryotic organism, including plants, fish, amphibians, reptiles, insects, yeast, fungi and mammals.