英和和英

名詞

benzonase (countable かつ uncountable, 複数形 benzonases)

1. (genetics) An artificial endonuclease used to degrade DNA and RNA.
   • 1991 October, J. M. Moreno, “Hydrolysis of nucleic acids in single-cell protein concentrates using immobilized benzonase”, in Applied Biochemistry and Biotechnology volume, volume 31:

     Hydrolysis of nucleic acids for single-cell protein concentrates has been carried out in one step using immobilized benzonase on corn cob.

   • 1999, Andrew J. Link, 2-d Proteome Analysis Protocols, page 83:

     The volume of benzonase solution (ready-made solution of the manufacturer) necessary to digest the DNA in the pellet III suspension was determined experimentally.

   • 2000 October, P. Ravindra, Anupama, Value-added food:: Single cell protein, in Biotechnology Advances, volume 18, issue 6, pages 459-479:

     Immobilized nucleases like benzonases on corncobs were also used to reduce the nucleic acid content in protein concentrates.

   • 2011, Charles Y. Lee, ‎Korkut Uygun, Methods in Bioengineering: Organ Preservation and Reengineering, page 185:

     Treat the lung with benzonase to remove the remnant DNA:

   • 2014 May 23, Muniasamy Neerathilingam, “Soni-removal of nucleic acids from inclusion bodies”, in Biochemical and Biophysical Research Communications, volume 448, number 1, page 45:

     Also, in many cases, enzymes like benzonases fail to completely remove nucleic acids from complexes due to inaccessibility of shorter nucleic acids buried in the refolded protein as demonstrated in this study.

   • 2015, Antonio Monari, ‎Elise Dumont, ‎Chryssostomos Chatgilialoglu, Radiation-induced and oxidative DNA damages, page 42:

     The new digestion protocol was designed based on the enzymatic combination of benzonase and P1 nucleases.