double stranded breakとは 意味・読み方・使い方

A mismatch sequence part of a double-stranded nucleic acid is cleaved and a variation in the nucleic acid is recognized, by making a double-stranded nucleic acid, having a mismatch sequence, react with a single-stranded nucleic acid cleaving nuclease in an alkaline buffer solution that contains a reagent to partly break a disulfide bond of the single-stranded nucleic acid cleaving nuclease.例文帳に追加

ミスマッチ配列を有する二本鎖核酸と一本鎖核酸切断ヌクレアーゼとを、一本鎖核酸切断ヌクレアーゼのジスルフィド結合を一部切断する試薬を含むアルカリ性緩衝液中で反応させて、二本鎖核酸に含まれるミスマッチ配列部分を切断し、核酸内の変異を認識する。 - 特許庁

A nucleic acid molecule is provided, comprising double-stranded fragments each with 24-100 bp, having at least one free end, and being a substrate for being bound by at least Ku protein involved in the NHEJ (non-homologous end joining) pathway of double-stranded break repair.例文帳に追加

２４〜１００bpの二本鎖部分を含み、少なくとも１つの自由端を有し、そして二本鎖切断修復のＮＨＥＪ経路に関与する少なくともＫｕタンパク質により結合するための基質である核酸分子。 - 特許庁

The methods and means are provided to improve targeted DNA insertion in plants using rare-cleaving "double stranded break" inducing enzymes.例文帳に追加

植物への標的指向ＤＮＡ挿入を改善するための、レア切断を起こす「二重鎖切断」誘導酵素を用いた方法および手段。 - 特許庁

The double-stranded nucleic acid fragments may interfere with DNA damage signaling and repair pathways, in particular the non-homologous NHEJ pathway of double-stranded break repair, and are put to use as adjuvant compositions to be used in conjunction with a DNA breaking treatment, particularly radiotherapy or chemotherapy.例文帳に追加

当該二本鎖核酸断片は、ＤＮＡ損傷シグナリング及び修復経路、特に二本鎖切断修復の非相同性ＮＨＥＪ経路を妨害することができ、ＤＮＡ切断処置、特に放射線治療又は化学療法と共同して使用されるべき佐剤組成物として使用される。 - 特許庁