出典:Wikipedia
出典:『Wikipedia』 (2011/07/02 23:52 UTC 版)
Phage display is a method for the study of protein–protein, protein–peptide, and protein–DNA interactions that uses bacteriophages to connect proteins with the genetic information that encodes them. Phage Display was originally invented by George P. Smith in 1985 and he demonstrated the display of peptides on filamentous phage by fusing the peptide of interest on to gene3 of filamentous phage. This technology was further developed and improved by groups at the MRC Laboratory of Molecular Biology with Winter and McCafferty and The Scripps Research Institute with Lerner and Barbas for display of proteins like antibodies for therapeutic protein engineering. The connection between genotype and phenotype enables large libraries of proteins to be screened and amplified in a process called in vitro selection, which is analogous to natural selection. The most common bacteriophages used in phage display are M13 and fd filamentous phage, though T4, T7, and λ phage have also been used.