single molecule fluorescent sequencingとは 意味・読み方・使い方

Single molecule fluorescent sequencing is one of massively parallel DNA sequencing, which has revolutionized many fields of biology by allowing the generation of sequence information on an unprecedented scale . Most of the sequence data generated thus far has been obtained with amplification-based sequencing systems , but single-molecule, non-amplification-based sequencing approaches are now possible, including at the scale of resequencing whole human genomes. It is based on the HelicosTM Genetic Analysis System, which consists of multiple components that work together as an integrated system. The fragments of DNA molecules are hybridized in place on disposable glass flow cells by using the Helicos Sample Loader in which the temperature can be adjusted for optimal hybridization. Each of the 25 channels on one standard flow cell can be addressed individually for addition of sample and any other needed sample preparation steps. Once the flow cells have been appropriately loaded with sample, they are inserted in the HeliScopeTM Sequencing System along with all the reagents necessary for sequencing by synthesis and imaging. The Sequencing System is then allowed to sequence as long as necessary with images being processed in real time by the HeliScope Analysis Engine. The Analysis Engine processes the images from each physical location and builds sequence reads from those images. Once the run is complete, the images processed, and strand formation complete, the data are downloaded to a compute cluster for reference alignment or assembly as needed.